ABSTRACT
Objectives: Stem cells are undifferentiated cells capable of creating different types of cell in the body. Stem cell proliferation often is performed in the culture medium supplemented with Fetal Bovine Serum [FBS]. Unknown compounds in the FBS, risk of contamination and disease transmission encourages the researches toward finding an alternative to FBS. Several factors are involved in the Mesenchymal Stem Cells [MSCs] precocious death in the transplanted tissue environment. Oxidative Stress [OS] is one of the main causes of stem cell apoptosis in the initial days after transplantation. The aim of this study was to evaluate the effect of Human Serum [HS] on the viability and oxidative related enzymes in human Adipose tissue-Derived Stem Cells [ADSCs] under oxidative stress in comparison with FBS
Materials and Methods: Human serum were obtained from blood of a healthy donor persons, in respective intervals during few days. The ADSCs were isolated from lipolysis operation samples and their cuture media were supplemented with FBS or HS and different concentrations of H2O2 as the oxidative agent
Results: The results showed that cell proliferation and viability of ADSCs under oxidative stress condition was significantly higher in the culture medium supplemented with HS in comparison with FBS supplemented medium [P<0.05]
Conclusion: This study showed that FBS could be replaced by HS in MSC culture medium with improved effects on cell proliferation and oxidative related enzyme activity under oxidative Stress condition
ABSTRACT
Aflatoxin Bj [AFBj] is a highly toxic and hepatocarcinogenic metabolite produced by Aspergillus species. Some natural products are known to kill fungi and destroy toxins and toxin-producing agents. The purpose of this study is to provide experimental data on the antifungal activity of cumin oils and their components that could be considered suitable for application in foods and drugs. The essential oil [EO] of Cuminum cyminum L. collected from Alborz Mountain, Iran, was obtained by hydro-distillation. The oil was analyzed by gas chromatography [GC] and chromatography/mass spectrophotometry [GC/MS]. The antifungal activity of the oil was studied with regard to the inhibition of the growth of Aspergillus flavus PICC-AF39 .Aspergillusflavus PlCC-AF24,Aspergillusparasiticus NRRL-2999 and Aspergillus niger. The minimal inhibitory [MIC] and minimal fungicidal [MFC] concentrations of the oil were determined. a-Pinene [29.2%], limonene [21.7%], 1,8-cineole [18.1%], linalool [10.5%], linalyl acetate [4.8%], and a-terpineole [3.17%]were the major components of the essential oil from C. cyminum L, and the oil showed a strong inhibitory effect on fungal growth. Essential oils could be safely used as preservatives in pharmaceuticals as well as health and food products to protect them against toxigenic fungal infections